| Component | Volume (μL) | Final Concentration (in 10 μL) |
|---|---|---|
| Superscript II first strand buffer (5×) | 2.00 | 1× |
| Betaine (5 M) | 2.00 | 1 M |
| Superscript II Reverse Transcriptase (200 U/μL) | 0.50 | 10 U/μL (100 U total) |
| DTT (0.1 M) | 0.50 | 5 mM |
| Nuclease-free sterile water | 0.29 | – |
| Recombinant RNase Inhibitor (40 U/μL) | 0.25 | 1 U/μL (10 U total) |
| LNA-TSO (100 μM) | 0.1 | 1 μM |
| MgCl2 (1 M) | 0.06 | 6 mM |
| Total Volume | 5.70 |
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