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. Author manuscript; available in PMC: 2019 Mar 18.
Published in final edited form as: Nat Protoc. 2017 Nov 16;12(12):2531–2553. doi: 10.1038/nprot.2017.120
Cycle Temperature (°C) Time Purpose
1 42 90 min RT and template switching
2–11 50 2 min Unfolding RNA secondary structure
42 2 min Continuation of RT and template switching
12 70 15 min Enzyme inactivation
13 4 Hold Safe storage