| Component | Volume (μL) | Final Concentration (in 25 μL) |
|---|---|---|
| KAPA HiFi HotStart ReadyMix (2×) | 12.50 | 1× |
| Nuclease-free sterile water | 2.25 | – |
| IS PCR primers (10 μM) | 0.25 | 0.1 μM |
| Total Volume | 15.00 |
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