Multimodal profiling of single-cell morphology, electrophysiology and gene expression using Patch-seq

. Author manuscript; available in PMC: 2019 Mar 18.

Published in final edited form as: Nat Protoc. 2017 Nov 16;12(12):2531–2553. doi: 10.1038/nprot.2017.120

Cycle	Temperature (°C)	Time	Purpose
1	98	3 min	Denature
2–19	98	20 s	Denature
	67	15 s	Anneal
	72	6 min	Extend
20	72	5 min	Extend
21	4	Hold	Safe storage