| Component | Volume (μL) | Final Concentration (in 25 μL) |
|---|---|---|
| 10× Tagmentation buffer | 1.00 | 1× |
| PEG-8000 (40% wt/vol) | 2.50 | 10% (wt/vol) |
| Tn5 Transposase (12.5 μM) | 0.10 | 1.25 μM |
| Nuclease-free sterile water | 0.40 | - |
| Sample DNA | 6.00 | 300 pg |
| Total Volume | 10.00 | - |
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