| Component | Volume (μL) | Final Concentration (in 25 μL) |
|---|---|---|
| KAPA HiFi Buffer (5×) | 5.00 | 1× |
| KAPA dNTP mix (10 mM each) | 0.75 | 0.3 mM each |
| Nuclease-free sterile water | 1.25 | – |
| KAPA enzyme (1U/μL) | 0.50 | 0.02 U/μL (0.5 U total) |
| Total Volume | 7.50 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.