Table 3.

Effect of fluoxetine and serotonin on the expression of mRNA for enamel proteins in LS8 cells

	Treatment time-point and drug concentration (μM)
	Day 1			Day 3			Day 7
Treatment group	0.1	1	10	0.1	1	10	0.1	1	10
Fluoxetine
Amel	92.1 ± 1.6	94.4 ± 0.8	84.7 ± 1.8*	94.9 ± 3.8	90.7 ± 0.2	72.7 ± 2.0*	102 ± 1.7	70.7 ± 6.8	83.9 ± 0.7*
MMP20	72.4 ± 2.5	70.7 ± 1.7	67.1 ± 1.2*	94.8 ± 0.7	98.7 ± 0.8	82.4 ± 3.3*	72.0 ± 3.2	73.5 ± 2.1	65.4 ± 3.9*
Enam	87.5 ± 1.2	79.2 ± 1.9	78.0 ± 1.0*	112.9 ± 1.7	103.5 ± 0.3	83.4 ± 3.8*	93.2 ± 1.2	87.3 ± 1.6	78.2 ± 1.5*
Ambn	100.7 ± 1.8	89.9 ± 1.5	94.7 ± 1.1	102.6 ± 1.2	96.1 ± 3.1	85.5 ± 0.9	92.6 ± 1.2	91.1 ± 1.3	95.5 ± 1.6
Serotonin
Amel	107.3 ± 1.5	91.0 ± 2.5	92.8 ± 0.88	85.7 ± 1.3	95.6 ± 0.8	104.9 ± 2.3	92.5 ± 3.6	105.4 ± 1.1	95.9 ± 1.4
MMP20	67.3 ± 0.3*	72.0 ± 2.1*	74.8 ± 1.4*	98.4 ± 3.0	83.1 ± 3.7	87.9 ± 1.7	76.2 ± 3.3*	68.1 ± 2.6*	74.4 ± 2.4*
Enam	90.8 ± 2.9	86.9 ± 0.8*	80.7 ± 1.4*	116.5 ± 1.0	120.4 ± 0.7	111.5 ± 3.7	87.2 ± 1.0*	84.6 ± 1.8*	87.5 ± 2.0*
Ambn	105.7 ± 0.2	94.4 ± 0.6	83.5 ± 4.4	111.2 ± 2.1	111.2 ± 1.4	101 ± 1.1	84.6 ± 1.9	86.4 ± 3.1	89.6 ± 1.6

Data are given as the mean ± SD of three biological replicates. Fluoxetine and serotonin were each analyzed at three different concentrations (0.1, 1.0, and 10 μM), and the results were determined at three time-points (days 1, 3, and 7).

Expression of mRNA was calculated relative to the expression of two housekeeping genes [β-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)], and the results are presented in percentage of untreated cells at each time-point.

^*P < 0.05.