Figure 7.

Stx17 N-peptide phosphorylation inhibits ectopic autophagosome clearance. a, representative single-channel and merged images of autophagic HeLa cells expressing EGFP-LC3 and the variant of mCherry-Stx17 are indicated. Both fusion-competent cells (left panels) and fusion-incompetent baf A₁–treated cells (right panels) are displayed. Scale bars, 10 μm. b and c, box plots of EGFP-LC3 (b) or mCherry-Stx17 (c) puncta concentration per cell in the presence or absence of baf A₁. d, box plots of Manders correlation coefficient to quantify colocalization between channels in a. Statistical significance tested with unpaired two-sample t tests (n = 10). ns, nonsignificant (p ≥ 0.05); *, p = 0.005–0.05; **, p = 0.0005–0.005; ***, p = 0.0001–0.0005; ****, p < 0.0001.