Figure 4.

POLQ-deficient cells are compromised in viability when ATR is inhibited. A, POLQWT and POLQKO-1 U2OS cells were infected with lentiviruses encoding ATR shRNA or the vector, and growth curves were plotted. B, POLQWT and POLQKO-1 U2OS cells were treated with ATR inhibitor VE-822 for 48 h at the indicated concentrations, and cell viability assays were performed. C, U2OS cells with or without POLQ shRNA-1 expression were treated with VE-822 for 48 h at the indicated concentrations, and cell viability assays were performed. The efficiency of POLQ knockdown is shown in Fig. 1C (right). In all experiments, error bars represent the S.D. of at least three independent experiments.