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. 2019 Jan 11;294(11):4012–4026. doi: 10.1074/jbc.RA118.006378

Figure 3.

Figure 3.

Effects of ASCT2 on cell migration. A, cell migration of 143B cells (ASCT2wt and ASCT2ko) was examined using a monolayer scratch-wound assay. Closure of the scratched areas was monitored over 24 h. Approximate cell boundaries are outlined in blue for clarity. Representative results of n = 8 experiments are shown. B, analysis of signal transduction pathways involved in cell migration. Focal adhesion kinase expression and phosphorylation of MLC (P-MLC) were detected by Western blotting in triplicate.