Table 3.
Source of antibodies and dilution for Western blotting
HRP, horseradish peroxidase; rpS6, ribosomal protein S6.
| Target | Manufacturer | Dilution | Molecular weight × 103 |
|---|---|---|---|
| ASCT1 (SLC1A4) | Cell Signaling Technology | 1:500 | 60–80 |
| ASCT2 (SLC1A5) | Cell Signaling Technology (D7C12) | 1:2,000 | 49–75 |
| SNAT1 (SLC38A1) | Millipore | 1:2,000 | 54 |
| SNAT2 (SLC38A2) | Abcam | 1:2,000 | 56 |
| LAT1 (SLC7A5) | Cell Signaling Technology | 1:1,000 | 39 |
| 4EBP1 | Cell Signaling Technology | 1:1,000 | 15–20 |
| Phospho-4EBP1 (Thr-37/46) | Cell Signaling Technology | 1:1,000 | 15–20 |
| eIF2a | Cell Signaling Technology | 1:1,000 | 38 |
| Phospho-eIF2a (Ser-51) | Cell Signaling Technology | 1:1,000 | 38 |
| rpS6 | Cell Signaling Technology | 1:1,000 | 32 |
| Phospho-rpS6 (Ser-240/244) | Cell Signaling Technology | 1:1,000 | 32 |
| ATF4 | Cell Signaling Technology | 1:1,000 | 49 |
| FAK | Cell Signaling Technology | 1:1,000 | 125 |
| MLC | Cell Signaling Technology | 1:1,000 | 18 |
| Phospho-MLC (Ser-19) | Cell Signaling Technology | 1:1,000 | 18 |
| Na/K-ATPase | Abcam | 1: 7,500 | 113 |
| Actin | Cell Signaling Technology | 1:5,000 | 45 |
| Rabbit IgG (HRP conjugate) | GE Healthcare | 1:1,000–1:10,000 | |
| Mouse IgG (HRP conjugate) | Cell Signaling Technology | 1:5,000–1:10,000 |