Figure 4.
Transcription of the copBL operon is regulated by CsoR. A, proposed CsoR-binding sites are shown for the S. aureus USA300_FPR3757 copA and copB promoter regions, as well as the B. subtilis copZ and S. aureus Newman copA promoter regions. B, transcriptional activity of copB is increased in a csoR mutant. Transcriptional activity of copB was monitored in the WT (JMB1100) and csoR::Tn (JMB6807) strains grown in chemically defined media containing 0 or 100 μm Cu. Fluorescence data were standardized to culture optical density (A590 nm). Data shown represent the average of biological triplicates with errors presented as standard deviations. Student's t test were performed on the data, and * indicates p ≤ 0.05, whereas N.S. denotes not significant.