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. 2019 Jan 17;294(11):3881–3898. doi: 10.1074/jbc.RA118.005050

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© 2019 Cho et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — Effects of overexpression of ANRIL (DQ485454) on EC functions and expression of cell adhesion molecules. A, relative expression of ANRIL (DQ485454) in HCAEC and EA.hy926 cells by transient transfection with plasmids, pcDNA3.1 or pcDNA3.1-ANRIL. B, monocyte adhesion to HCAEC and EA.hy926 cells transfected with plasmids, pcDNA3.1 or pcDNA3.1-ANRIL (n = 5). C, TEM with HCAEC and EA.hy926 cells transfected with plasmids, pcDNA3.1 or pcDNA3.1-ANRIL (n = 5). D, migration of HCAEC and EA.hy926 cells transfected with plasmids, pcDNA3.1 or pcDNA3.1-ANRIL (n = 5). E, Western blot analysis of cell adhesion proteins (ICAM-1, ICAM-2, VCAM-1, E-selectin, P-selectin, pVE-Cad, and tVE-Cad) using HCAECs transfected with plasmids, pcDNA3.1 or pcDNA3.1-ANRIL (n = 3). Tubulin was used as a loading control. F, Western blotting images in E were quantified and plotted. Data were normalized to the baseline Tubulin expression, which was defined as 1.0. *, p < 0.05; **, p < 0.01. Only statistically significant differences are marked with asterisks. Error bars, S.D.