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. 2019 Jan 17;294(11):3881–3898. doi: 10.1074/jbc.RA118.005050

Figure 3.

Figure 3.

Effects of knockdown of ANRIL (DQ485454) on EC functions and expression of cell adhesion molecules. A, relative expression of ANRIL in HCAECs and EA.hy926 cells transiently transfected with either siNC or siANRIL. B, relative quantification of monocyte adhesion to HCAEC and EA.hy926 cells transfected with siNC or siANRIL (n = 5). C, relative quantification of TEM with HCAEC and EA.hy926 cells transfected with siNC or siANRIL (n = 5). D, migration of HCAEC and EA.hy926 cells transfected with siNC or siANRIL (n = 5). E, Western blot analysis of cell adhesion proteins (ICAM-1, ICAM-2, VCAM-1, E-selectin, P-selectin, pVE-Cad, and tVE-Cad) using HCAECs transfected with siNC or siANRIL (n = 3). Tubulin was used as a loading control. F, Western blotting images in E were quantified and plotted. Data were normalized to the baseline tubulin expression, which was defined as 1.0. *, p < 0.05; **, p < 0.01. Only statistically significant differences are marked with asterisks. Error bars, S.D.