Figure 5.

Validation of downstream genes of ANRIL from global microarray gene expression analysis using semi-quantitative RT-PCR and real-time RT-PCR analyses. A, semi-quantitative RT-PCR analysis using HUVECs transfected with siNC or siANRIL (n = 3, only two shown). The top 16 protein-coding genes from the microarray analysis were selected for validation analysis. Genes were aligned by their relative -fold changes in siNC versus siANRIL. GAPDH was used as an internal control. B, real-time qRT-PCR analysis to validate ANRIL-regulated genes using HUVECs transfected with siNC or siANRIL (n = 3). Data were normalized to the baseline GAPDH expression, which was defined as 1.0. *, p < 0.05; **, p < 0.01. Only statistically significant differences are marked with asterisks. Error bars, S.D.