Figure 1.
NanoLuc complementation system. A, constructs for the NanoLuc complementation assay between a GPCR linked to SmBiT, NP, or HiBiT peptides and a Gα subunit linked to LgBiT. Upon stimulation (yellow circle), the coupling of heterotrimeric G protein to the receptor triggers the exchange of a GDP (purple star) by a GTP (green star) followed by dissociation of Gα from Gβγ subunits. The interaction between GPCR and Gα subunit induces the formation of a complete NanoLuciferase and light emission in the presence of its substrate. B, amino acid sequence and KD value of the different small peptides (SmBiT, NP, and HiBiT). Red amino acids represent mutated amino acids compared to NP.