Figure 3.
Reversibility of the SmBiT, NP, and HiBiT peptides. NanoLuc activity was measured in HEK293 cells co-expressing Gαi1–LgB91 and D2 linked to SmBiT (A), NP (B), or HiBiT (C), before and after stimulation with the receptor ligand dopamine (1 μm). Twenty minutes after addition of agonist, a selective-receptor antagonist sulpiride (10 μm) was injected. Data represent stimulated cells normalized to nonstimulated cells. Data are representative of the mean ± S.E. of at least three independent experiments.