Figure 4.

NanoLuc complementation measurement of GPCR and G protein interactions in living cells. A–D, NanoLuc activity was measured in HEK293 cells co-expressing Gα subunit linked to LgBiT and GPCR linked to NP as indicated in each panel. Cells were stimulated with vehicle or with their respective agonist (isoproterenol, histamine, and dopamine 10 μm; U46619 10 nm), and the results are expressed as the normalization of agonist-treated cells to untreated cells. Data represent the mean ± S.D. of three independent experiments performed in triplicate. Statistical significance between stimulated and unstimulated cells was assessed using a nonparametric Mann-Whitney test (***, p < 0.001).