Figure 1.

Multiple-sequence alignments of GH30 xylanases. Primary structures of Xyn30B, XYN IV, XYN VI, EcXynA, and BsXynC were used for sequence alignments. The features are shown as follows: conserved Arg residues in GH30-8 glucuronoxylanases (red box); catalytic Glu residues (highlighted in gray); predicted signal sequence of Xyn30B (highlighted in black); β2–α2 loop of Xyn30B and EcXynA (boldface type); N-glycosylated Asn residues (red characters); the conserved Arg residues in GH30-7 glucuronoxylanases (highlighted in red); Asn⁹³ of Xyn30B (indicated by a black arrow). Secondary structures of Xyn30B are shown above the sequences. Each of the secondary structures of EcXynA and BsXynC is also indicated within the sequence. N-terminal signal sequence, N-glycosylation sites, and secondary structures are based on assignment by the crystal structure.