Figure 2.

Cytoplasmic Hsp70s, Hsp90s, Hsp40s, chaperonins, and Hip were immunoprecipitated together with the soluble CD38. a, HEK-293T cells stably expressing FLAG-sCD38, or FLAG-EGFP as a negative control, were permeabilized by 100 μm digitonin and the cytosolic fractions were subjected to IP with anti-FLAG M2 Magnetic Beads, eluted by 3×FLAG peptide and analyzed by SDS-PAGE. b, the information of the chaperones identified by LC-MS/MS. Family: name of the protein family; Protein: reference name of protein documented in GenBank, NCBI; Accession: accession number in UniProt; Protein NM: molecular mass of the proteins; Coverage: percentage of the protein sequence that was covered by identified peptides; No. of peptides: number of peptide sequences unique to the identified proteins. c, Western blot analysis of both the immunoprecipitation (left panel) and input (right panel) by the antibodies labeled.