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. 2019 Jan 22;294(11):3987–3999. doi: 10.1074/jbc.RA118.007221

Figure 6.

Figure 6.

Extracted ion chromatograms for trypsin-digested peptides from HT-CpeBA coexpressions. Combined extracted ion chromatograms for unmodified Cys-48/Cys-59 peptides (red) and PEB-cross-linked versions of Cys-48/Cys-59 peptides (blue and indicated with arrows) from purified HT-CpeBA obtained from E. coli cells expressing pCpeBA and pNT-PebS in addition to pCpeS (BA+S+PEB) (A), pCpeF2 and pNT-CpeZ3 (BA+F+Z+PEB) (B), pCpeSF2 and pNT-CpeZ3 (BA+SF+Z+PEB) (C), or pCpeSF2 and pNT-CpeYZ (BA+SF+YZ+PEB) (D) are shown. These results are representative of two independent replicates.