Figure 2.

Validation of activators, concentration response. Verified hits were assigned into drug classes based on shared structures and targets (Table S1). Representative compounds from each group were chosen for further analysis. For confirmation, representative hits were confirmed by ordering pure compound and rescreening. For concentration response, cells were pretreated with insulin (0.3 nm), and compounds were added at increasing concentrations, and cells were incubated for 1 h. Cells were placed on ice, and cell-surface Glut4 was determined. A, insulin sensitizers and dis-inhibitors. B, β-adrenergic receptor agonists. C, glucocorticoids. To verify targets, the measured EC₅₀ values (Table S1) were compared with published values.