Figure 6.

Validation of inhibitors, concentration response. Verified hits were assigned into drug classes based on shared structures and targets (Table S1). Representative hits were confirmed using pure compound and validated by dose response. A, drugs with known targets/mechanisms of action. Note: IBMX (EC₅₀ = 0.5 mm) is acting via adenoreceptor inverse agonism and not through effects on cAMP as a phosphodiesterase inhibitor (IC₅₀ PDE = 2–50 μm). Because of its low affinity, IBMX would not have screened positive as an inhibitor (25 μm). B, inhibitors identified in the screen with unknown mechanism of action.