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Kinetics of reduction of ferryl heme in solutions of sickle-cell hemoglobin mutants by ascorbate. A, autoreduction of ferryl hemoglobin in PBS at room temperature as monitored in the stopped flow. B, reduction of ferryl heme (5 μm) after mixing with 50 μm ascorbate in PBS at room temperature and monitoring spectral changes with a stopped-flow spectrophotometer.
