Figure 8.

Kinetics of polymerization and heat stability of hemoglobin A, hemoglobin S, and its mutants. A, kinetics of deoxy-Hb polymer formation. Solutions of deoxy-Hb (100 μm) in 1.8 m phosphate buffer were rapidly heated from 0 to 30 °C, and polymer formation was assessed by light scattering at 700 nm. B, various mutants were heated at 60 °C for 10 min in 100 mm phosphate, pH 7.4, and the percent of precipitated hemoglobin was calculated after centrifugation. Turbidity was also measured spectrophotometrically by recording the absorbance at 700 nm of the concentrated hemoglobin solutions (2 mm).