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. 2019 Jan 10;294(11):4145–4159. doi: 10.1074/jbc.RA118.006452

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Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Figure 9. — Typical subset of tryptic digestion profiles (base peak chromatograms) for human hemoglobins S and A (controls) and the hemoglobin S mutant (E6V/F41Y) after peroxide treatment. Base peak chromatograms listed for HbA, native HbS, rHbS, rHbS βF41Y, and rHbS βF41Y/K82D after parallel experimental treatment with 2.5× H₂O₂. Each chromatogram shows similar elution peaks detected during MS analysis. The similarity in peaks and retention times indicates and validates trypsin digestion efficiency for each run.