Table 3.
Hydrogen peroxide-mediated oxidation of the cysteine groups in hemoglobin S mutants
| βCys-93 oxidation |
βCys-112 oxidation |
αCys-104 oxidation |
||||
|---|---|---|---|---|---|---|
| 2.5× H2O2 | 10× H2O2 | 2.5× H2O2 | 10× H2O2 | 2.5× H2O2 | 10× H2O2 | |
| HbA | 22.1 ± 1.3% | 51.1 ± 2.5% | 2.9 ± 0.8% | 19.5 ± 1.5% | 1.6 ± 0.4% | 7.9 ± 1.4% |
| Native HbS | 34.9 ± 2.2% | 62.4 ± 2.9% | 17.6 ± 1.4% | 25.2 ± 2.3% | 5.4 ± 1.1% | 11.7 ± 0.6% |
| rHbS | 37.4 ± 1.5% | 61.7 ± 0.7% | 18.4 ± 1.0% | 27.2 ± 2.1% | 6.6 ± 0.9% | 12.8 ± 0.8% |
| rHbS βF41Y | 33.7 ± 3.5% | 57.3 ± 1.7% | 19.6 ± 1.9% | 23.0 ± 2.3% | 4.8 ± 0.8% | 12.0 ± 1.3% |
| rHbS βK82D | 11.9 ± 2.0% | 22.1 ± 1.3% | 20.8 ± 1.5% | 23.4 ± 0.4% | 6.2 ± 0.8% | 10.7 ± 0.8% |
| rHbS βF41Y/K82D | 4.6 ± 0.6% | 9.1 ± 1.1% | 19.7 ± 0.7% | 25.4 ± 3.3% | 6.6 ± 2.2% | 11.4 ± 0.4% |