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. 2019 Mar 11;9:49. doi: 10.3389/fcimb.2019.00049

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Copyright © 2019 Imperi, Fiscarelli, Visaggio, Leoni and Visca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of 5-fluorocytosine (5-FC) and 5-fluorouracil (5-FU) (0–1,000 μM) on growth and relative pyoverdine production (upper and lower panel, respectively) of P. aeruginosa PAO1 and five isogenic spontaneous mutants resistant to 5-FU (R1–R5) cultured in TSBD for 14 h in microtiter plates at 37°C. Values represent the mean (±SD) of three independent experiments. Asterisks indicate statistically significant differences with respect to the corresponding untreated control (*P < 0.05, ***P < 0.001; ANOVA).