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. 2019 Mar 11;9:49. doi: 10.3389/fcimb.2019.00049

Figure 5.

Figure 5

Effect of 5-fluorocytosine (5-FC) and 5-fluorouracil (5-FU) at 40 or 400 μM on the competitive fitness of spontaneous 5-FU resistant mutants of P. aeruginosa PAO1. Each 5-FU resistant isolate (R1, R2, R3, R4, or R5) and the parental strain PAO1 were precultured in TSBD with 50 μM FeCl3 and then inoculated at 1:1,000 final dilution in TSBD in a 1:100 ratio (5-FU R:WT). Percentage of 5-FU resistant cells with respect to 5-FU sensitive ones was evaluated after 16-h incubation at 37°C by plating on SM9 agar plates with or without 120 μM 5-FU. Values represent the mean (±SD) of ten independent experiments (two for each 5-FU resistant isolate). Asterisks indicate statistically significant differences (*P < 0.05, **P < 0.01, ***P < 0.001; ANOVA).