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. 2019 Mar 11;9:49. doi: 10.3389/fcimb.2019.00049

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Copyright © 2019 Imperi, Fiscarelli, Visaggio, Leoni and Visca.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Box plots with Turkey whiskers showing the effect of 5-fluorocytosine (5-FC) and 5-fluorouracil (5-FU) at 10 or 100 μM on growth (A) and pyoverdine production (B) in a collection of 97 (A) or 80 (B) P. aeruginosa CF isolates cultured in TSBD until the late-exponential growth phase. Data are expressed as percentage with respect to untreated cultures (100%). For each isolates, values are the mean of two or three independent assays. Of the 100 isolates originally included in the collection, 3 did not grow and 17 did not produce detectable amounts of pyoverdine under the conditions tested, and were not included in the plots shown in (A,B), respectively. Black dots represent the outliers. Asterisks indicate statistically significant differences (**P < 0.01, ***P < 0.001; Kruskal-Wallis).