Table 2.
Evaluation of real-time PCR for detection and differentiation of C. albicans and C. dubliniensis among 226 clinical Candida isolates speciated by Vitek 2 yeast identification system
| Candida species by Vitek 2 | Clinical isolates tested, n | Tm by melting curve analysis (mean + SD), ° C | C. albicans or C. dubliniensis isolates correctly detected by real-time PCR, n | Isolates sequenced, n |
|---|---|---|---|---|
| C. albicans | 113 | 86.680±0.529 | 113 | 73 |
| C. dubliniensis | 98 | 82.616±0.535 | 98 | 98 |
| C. glabrata | 2 | no amplification detected | not applicable | 2 |
| C. parapsilosis | 2 | no amplification detected | not applicable | 2 |
| C. orthopsilosis | 2 | no amplification detected | not applicable | 2 |
| C. tropicalis | 2 | no amplification detected | not applicable | 2 |
| C. lusitaniae | 2 | no amplification detected | not applicable | 2 |
| C. krusei | 2 | no amplification detected | not applicable | 2 |
| C. haemulonii | 1 | no amplification detected | not applicable | 1 |
| C. famata | 1 | no amplification detected | not applicable | 1 |
| C. kefyr | 1 | no amplification detected | not applicable | 1 |