Table 2.
Table of changes in proteomic profile in IC following acute H2S exposure.
| Gene names | Protein names | GOBP name | 2h |
Day 2 |
Day 4 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fold change | Avg | sthev | t-Test p | Fold change | Avg | sthev | t-Test p | Fold change | Avg | sthev | t-Test p | |||
| Arpc4 | Actin-related protein 2/3 complex subunit 4 | Actin cytoskeleton organization | Up | 0.32 | 0.08 | 0 | Down | −0.26 | 0.05 | 0 | 0 | 0.06 | 0 | |
| Dstn | Destrin | Actin cytoskeleton organization | 0 | 0.11 | 0 | Down | −0.52 | 0.3 | 0.0013 | Up | 0.28 | 0.12 | 0.0004 | |
| Iqsecl | IQ motif and SEC7 domain-containing protein 1 | Actin cytoskeleton organization | Down | −0.33 | 0.24 | 0.0006 | ||||||||
| Kif2a | Kinesin-like protein | Actin cytoskeleton organization | Down | −0.28 | 0.1 | 0 | ||||||||
| Trf | Serotransferrin | Actin cytoskeleton organization | 0.26 | 0.18 | 0.0003 | 0.25 | 0.18 | 0.0002 | Up | 0.29 | 0.06 | 0 | ||
| Lmna | Prelamin-A/C | Adhesion | Up | 0.36 | 0.13 | 0.0004 | ||||||||
| Vim | Vimentin | Adhesion | Up | 0.44 | 0.24 | 0.0009 | ||||||||
| Eprs | Bifunctional glutamate/proline-tRNA ligase | Amine metabolic process | Down | −0.28 | 0.16 | 0.0001 | ||||||||
| Vdac1 | Voltage-dependent anion-selective channel protein 1 | Anion transport | −0.26 | 0.08 | 0 | −0.08 | 0.08 | 0 | Down | −0.27 | 0.19 | 0.0015 | ||
| Gdap1l1 | Ganglioside-induced differentiation-associated protein 1-like 1 | Axon guidance | Down | −0.27 | 0.1 | 0 | 0 | 0.11 | 0 | −0.05 | 0.06 | 0 | ||
| Dpysl4 | Dihydropyrimidinase-related protein 4 | Axon guidance | Down | −0.35 | 0.19 | 0.0003 | ||||||||
| Nef1 | Neurofilament light polypeptide | Axon guidance | Down | −0.36 | 0.14 | 0.0001 | −0.19 | 0.18 | 0.0002 | 0.03 | 0.09 | 0.0001 | ||
| Plp1 | Myelin proteolipid protein | Axon guidance | Down | −0.38 | 0.37 | 0.0031 | −0.04 | 0.38 | 0.0042 | Down | −0.36 | 0.23 | 0.0025 | |
| Alb | Serum albumin | Blood | Up | 0.37 | 0.16 | 0.0002 | Up | 0.42 | 0.17 | 0.0002 | Up | 0.55 | 0.12 | 0.0003 |
| Hbbt1 | Hemoglobin subunit beta-2 | Blood | Up | 0.45 | 0.4 | 0.0069 | Up | 0.33 | 0.23 | 0.0007 | Up | 0.49 | 0.32 | 0.0066 |
| Slc2a1 | Solute carrier family 2, facilitated glucose transporter member 1 | Carbohydrate transport | Down | −0.28 | 0.34 | 0.0024 | 0.01 | 0.36 | 0.0034 | Down | −0.37 | 0.24 | 0.0029 | |
| Cend1 | Cell cycle exit and neuronal differentiation protein 1 | Cell cycle | −0.24 | 0.16 | 0.0002 | −0.13 | 0.28 | 0.0012 | Up | 0.28 | 0.08 | 0.0001 | ||
| Dnah6 | Dynein Axonemal Heavy Chain 6 | Cell motility | Up | 0.41 | 0.16 | 0.0002 | ||||||||
| Ehd3 | EH domain-containing protein 3 | Cellular transport | 0.01 | 0.14 | 0.0001 | −0.24 | 0.06 | 0 | Down | −0.21 | 0.28 | 0.0045 | ||
| Exoc1 | Exocyst complex component 1 | Cellular transport | Down | −0.26 | 0.12 | 0 | 0.01 | 0.17 | 0.0002 | 0.08 | 0.07 | 0.0001 | ||
| Lgi3 | Leucine-rich repeat LGI family member 3 | Cellular transport | −0.11 | 0.17 | 0.0002 | 0.07 | 0.07 | 0 | Up | 0.28 | 0.06 | 0.0001 | ||
| H3f3a | Histone H3.3 | DNA metabolism | Down | −0.34 | 0.29 | 0.0014 | Down | −0.29 | 0.22 | 0.0005 | −0.24 | 0.21 | 0.0018 | |
| Hist1h3a | Histone H3.1 | DNA metabolism | −0.09 | 0.1 | 0 | Down | −0.34 | 0.17 | 0.0002 | −0.21 | 0.4 | 0.0139 | ||
| Set | Protein SET | DNA metabolism | Up | 0.33 | 0.22 | 0.0006 | −0.07 | 0.09 | 0 | −0.11 | 0.09 | 0.0002 | ||
| Atp5j2 | ATP synthase subunit f, mitochondrial | Energy metabolism | −0.09 | 0.08 | 0 | Down | −0.52 | 0.1 | 0 | 0.04 | 0.07 | 0.0001 | ||
| Ndufab1 | NADH:Ubiquinone Oxidoreductase Subunit AB1 | Energy metabolism | −0.2 | 0.11 | 0 | Down | −0.3 | 0.18 | 0.0002 | −0.04 | 0.02 | 0 | ||
| Cox6c | Cytochrome c oxidase subunit 6C | Energy metabolism | −0.09 | 0.15 | 0.0001 | −0.06 | 0.09 | 0 | Down | −0.44 | 0.22 | 0.0021 | ||
| Atp6v1g2 | V-type proton ATPase subunit G 2 | Energy metabolism | Down | −0.36 | 0.11 | 0 | Down | −0.31 | 0.31 | 0.0061 | ||||
| Ndufa13 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 | Energy metabolism | −0.1 | 0.07 | 0 | −0.26 | 0.09 | 0 | Down | −0.33 | 0.12 | 0.0003 | ||
| Atp6v1h | V-type proton ATPase subunit H | Energy metabolism | Down | −0.49 | 0.21 | 0.0004 | −0.04 | 0.07 | 0 | −0.01 | 0.02 | 0 | ||
| Idh3a | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial | Energy metabolism | Down | −0.28 | 0.04 | 0 | 0.04 | 0.08 | 0 | 0.07 | 0.05 | 0 | ||
| Mtco2 | Cytochrome c oxidase subunit 2 | Energy metabolism | Down | −0.34 | 0.15 | 0.0001 | −0.04 | 0.1 | 0 | −0.14 | 0.12 | 0.0003 | ||
| Atp6v1g1 | V-type proton ATPase subunit G 1 | Energy metabolism | Up | 0.56 | 0.22 | 0.0022 | ||||||||
| Me1 | Malic enzyme | Energy metabolism | Down | −0.53 | 0.09 | 0 | ||||||||
| Ogdhl | Oxoglutarate Dehydrogenase-Like | Energy metabolism | 0.09 | 0.23 | 0.0006 | Up | 0.3 | 0.11 | 0.0003 | |||||
| Oxct1 | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial | Energy metabolism | −0.01 | 0.07 | 0 | 0.06 | 0.07 | 0 | Up | 0.48 | 0.1 | 0.0002 | ||
| Hsd17b10 | 3-hydroxyacyl-CoA dehydrogenase type-2 | Fatty acid and steroid metabolism | −0.24 | 0.14 | 0.0001 | Down | −0.46 | 0.18 | 0.0012 | |||||
| Gpd2 | Glycerol-3-phosphate dehydrogenase | Glucose metabolism | −0.01 | 0.15 | 0.0001 | Up | 0.27 | 0.09 | 0 | 0.2 | 0.19 | 0.0013 | ||
| Gpi | Glucose-6-phosphate isomerase | Glucose metabolism | Down | −0.46 | 0.13 | 0.0001 | −0.23 | 0.07 | 0 | −0.1 | 0.01 | 0 | ||
| Pfkp | ATP-dependent 6-phosphofructokinase | Glucose metabolism | Down | −0.35 | 0.1 | 0 | 0.12 | 0.1 | 0 | 0.05 | 0.05 | 0 | ||
| Pgam2 | Phosphoglycerate mutase 2 | Glucose metabolism | Down | −0.39 | 0.1 | 0 | ||||||||
| C1qbp | Complement component 1 Q subcomponent-binding protein, mitochondrial | Immune response | 0.03 | 0.21 | 0.0004 | 0.17 | 0.1 | 0 | Up | 0.34 | 0.08 | 0.0001 | ||
| Mif | Macrophage migration inhibitory factor | Immune response | Down | −0.35 | 0.16 | 0.0001 | −0.07 | 0.07 | 0 | 0 | 0.16 | 0.0008 | ||
| Acat2 | Acetyl-CoA acetyltransferase, cytosolic | Lipid metabolism | 0.03 | 0.08 | 0 | −0.02 | 0.24 | 0.0007 | Up | 0.34 | 0.09 | 0.0002 | ||
| Tuba1b | Tubulin alpha-1B chain | Microtubule organization | Down | −0.28 | 0.09 | 0 | −0.25 | 0.09 | 0 | Down | −0.31 | 0.08 | 0.0001 | |
| Tubb6 | Tubulin beta-6 chain | Microtubule organization | Down | −0.67 | 0.1 | 0.0002 | ||||||||
| Mp68 | 6.8 kDa mitochondrial proteolipid | Mitochondrial proteolipid | Down | −0.35 | 0.09 | 0 | Down | −0.4 | 0.15 | 0.0007 | ||||
| Slc25a51 | Solute carrier family 25 member 51 | Mitochondrial transport | Up | 0.35 | 0.1 | 0 | ||||||||
| Sccpdh | Saccharopine dehydrogenase-like oxidoreductase | oxidoreductase | down | −0.4 | 0.11 | 0 | ||||||||
| Pcyt2 | Ethanolamine-phosphate cytidylyltransferase | Phosphatidylethanolamine biosynthesis | Down | −0.3 | 0.13 | 0.0004 | ||||||||
| Psmc4 | 26S protease regulatory subunit 6B | Protein degradation | Down | −0.51 | 0.12 | 0 | ||||||||
| Ahsa1 | Activator of 90 kDa heat shock protein ATPase homolog 1 | Protein folding | Down | −0.59 | 0.22 | 0.0004 | Down | −0.35 | 0.15 | 0.0001 | −0.06 | 0.08 | 0.0001 | |
| Hsbp1 | Heat shock factor-binding protein 1 | Protein folding | Down | −0.3 | 0.1 | 0 | ||||||||
| Hspa4l | Heat shock 70 kDa protein 4 L | Protein folding | Down | −0.43 | 0.03 | 0 | −0.2 | 0.21 | 0.0004 | |||||
| Gm8797 | Ubiquitin-60S ribosomal protein L40 | RNA metabolism | Up | 0.46 | 0.41 | 0.0078 | Up | 0.47 | 0.13 | 0.0001 | 0.16 | 0.13 | 0.0005 | |
| Hnrnpd | Heterogeneous nuclear ribonucleoprotein D0 | RNA metabolism | Up | 0.3 | 0.15 | 0.0001 | −0.1 | 0.12 | 0.0004 | |||||
| Pcbp1 | Poly(rC)-binding protein 1 | RNA metabolism | Down | −0.49 | 0.11 | 0 | −0.16 | 0.1 | 0 | −0.2 | 0.14 | 0.0006 | ||
| Pabpc2 | Polyadenylate-binding protein | RNA metabolism | Down | −0.29 | 0.16 | 0.0001 | ||||||||
| Hnrnpl | Heterogeneous nuclear ribonucleoprotein L | RNA metabolism | Down | −0.4 | 0.29 | 0.0013 | ||||||||
| Rps20 | 40S ribosomal protein S20 | RNA metabolism | Down | −0.31 | 0.13 | 0.0005 | ||||||||
| Rp17 | 60S ribosomal protein L7 | RNA metabolism | Down | −0.45 | 0.13 | 0.0001 | −0.08 | 0.08 | 0 | −0.16 | 0.07 | 0.0001 | ||
| Pcbp2 | Poly(rC)-binding protein 2 | RNA metabolism | Down | −0.63 | 0.17 | 0.0002 | 0.02 | 0.03 | 0 | 0.17 | 0.04 | 0 | ||
| Git1 | ARF GTPase-activating protein GIT1 | Signaling | 0.04 | 0.08 | 0 | Up | 0.34 | 0.13 | 0.0001 | |||||
| Arf3 | ADP-ribosylation factor 3 | Signaling | −0.03 | 0.05 | 0 | −0.09 | 0.08 | 0 | Down | −0.38 | 0.1 | 0.0002 | ||
| Arhgap35 | Rho GTPase-activating protein 35 | Signaling | Down | −0.27 | 0.22 | 0.0005 | ||||||||
| Gnaq | Guanine nucleotide-binding protein G(q) subunit alpha | Signaling | Down | −0.3 | 0.22 | 0.0004 | −0.17 | 0.12 | 0 | −0.07 | 0.06 | 0 | ||
| Rhot1 | Mitochondrial Rho GTPase 1 | Signaling | Down | −0.33 | 0.06 | 0 | ||||||||
| Rab1 | Ras-related protein Rab-1A | Signaling | Down | −0.27 | 0.14 | 0.0001 | 0 | 0.09 | 0 | 0.14 | 0.07 | 0.0001 | ||
| Plaa | Phospholipase A-2-activating protein | Signaling | Down | −0.69 | 0.25 | 0.0006 | ||||||||
| Prkacb | cAMP-dependent protein kinase catalytic subunit beta | Signaling | Up | 0.53 | 0.29 | 0.0021 | −0.05 | 0.08 | 0 | −0.12 | 0.09 | 0.0001 | ||
| Synj1 | Synaptojanin-1 | Synaptic transmission | Down | −0.38 | 0.16 | 0.0001 | 0.16 | 0.09 | 0.0001 | |||||
| Cldn11 | Claudin-11 | Tight junction component | −0.21 | 0.19 | 0.0003 | Down | −0.46 | 0.23 | 0.0006 | −0.11 | 0.13 | 0.0005 | ||
| Eef1b2 | Elongation factor 1-beta | Translation | Down | −0.29 | 0.19 | 0.0003 | 0.21 | 0.11 | 0.0002 | |||||
| Napb | Beta-soluble NSF attachment protein | Vesicle transport | Down | −0.38 | 0.06 | 0 | −0.09 | 0.06 | 0 | −0.02 | 0.04 | 0 | ||
| Dnm3 | Dynamin-3 | Vesicle transport | Down | −0.38 | 0.13 | 0.0001 | 0.08 | 0.05 | 0 | |||||
| Vps26b | Vacuolar protein sorting-associated protein 26B | Vesicle transport | Up | 0.31 | 0.17 | 0.000223 | ||||||||
| Sv2a | Synaptic vesicle glycoprotein 2A | Vesicle transport | −0.21 | 0.12 | 0.0001 | 0.08 | 0.1 | 0 | Up | 0.29 | 0.16 | 0.0008 | ||
Table displays the Perseus processed protein list containing gene names, protein names, GO biological process names, one-sample t-test, and change of significantly modulated proteins on 2 h and days 2 and 4. This table is complimentary to Morpheus rendered Heatmap of determined changes in protein expression on 2 h and days 2 and 4 (Fig. 4).