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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Circ Heart Fail. 2019 Mar;12(3):e005234. doi: 10.1161/CIRCHEARTFAILURE.118.005234

Figure 2. Doxorubicin causes cardiomyocyte atrophy in vivo.

Figure 2.

Mice were sacrificed 7 days after receiving doxorubicin 20 mg/kg i.p. (A) Representative wheat germ agglutinin-Alexa Fluor 488 conjugate stained heart sections. Analysis of myocyte cross-sectional areas from 4 hearts (800 cross-sectional areas total, 200 cross sections per mouse over 4–6 histological sections). A Student’s t-test was used to determine significance between groups. (B) Serum Troponin I was measured by ELISA. (C) Summary densitometry of immunoblots for markers of apoptosis. n= 6 for VC, n = 3–4 for DOX-treated. Results were compared using a one-way ANOVA. c-Caspase 3 = cleaved caspase 3; c-PARP = cleaved poly ADP ribose polymerase, LAD = left anterior descending coronary artery.