Figure 4. Doxorubicin increases cardiac expression of MuRF-1 and a molecular signature of atrophy in vivo.

Mice were sacrificed 7 days after intraperitoneal injection of doxorubicin or vehicle control. (A) Quantitative RT-PCR assayed molecular markers of atrophy at various DOX doses. Logistic regression was applied to MuRF1 results. (B) Heart lysates were immunoblotted and (C) summary densitometry was compared (vehicle control vs. DOX 25 mg/kg) by Student’s t-test.