Tumor cells within hemangiosarcoma metastases demonstrate positive immunolabeling for CCL2. All evaluated cases (n=5) demonstrated strong, positive immunolabeling for CCL2. (A) In all cases, greater than 50-75% of tumor cells within hemangiosarcoma pulmonary metastases demonstrated moderate to strong immunoreactivity for CCL2. 20x magnification. DAB chromogen. Hematoxylin counterstain. (B) Higher magnification image of the same case in (A), which demonstrates the strongly positive, intra-cytoplasmic, peri-nuclear (golgi zone) localization for CCL2 within tumor cells. 50x magnification.