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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Hypertension. 2019 Apr;73(4):829–838. doi: 10.1161/HYPERTENSIONAHA.118.12286

Figure 1. Ang-II infusion is associated with elevated platelet counts, increased levels of immature platelet and a shortened platelet life-span.

Figure 1.

Wild type (WT) mice were implanted with either Ang-II (1 μg/kg/min) or control (saline) loaded micro-osmotic mini pumps for up to 14 days. Flow cytometry was performed to quantify platelet counts, immature platelet levels, newly released platelet levels and platelet life-span. A) Total platelet levels and B) immature platelets levels after 1 week of Ang-II infusion (expressed as a platelet number per μl of whole blood on day 0, 7, 10 and 14). C) Platelets produced following Ang-II infusion were monitored after day 7 and numbers of newly released platelets (biotin negative: CD41+SA−) were quantified daily. D) Platelet lifespan was determined in saline (WT) and Ang-II-infused groups (expressed in hours). Data are mean ± SEM of 3–6 mice per group. *p < 0.05, **p < 0.01 vs. WT mice.