Figure 5.
Effect of stable overexpression and knockdown CDK11p110 in ESCC cell lines. (a) EC109 and KYSE150 cell viability was evaluated for 72 h using a CCK8 assay after infection with lentiviruses expressing the pLKO.1 vector, pLKO.1-shCDK11p110-1 or pLKO.1-shCDK11p110-2 and puromycin selection for 7 days. ***P < 0.001 compared with the pLKO.1 group. (b) CDK11p110 expression was detected by western blot assay in stable CDK11p110 knockdown EC109 and KYSE150 cell lines. (c) EC109 and KYSE150 cell proliferation was evaluated at the indicated time points using a CCK8 assay after infection with lentiviruses expressing the pCDH vector or pCDH-CDK11p110 and puromycin selection for 7 days. *P < 0.05 compared with the pCDH-vector group. (d) CDK11p110 expression was detected by western blot assay in stable CDK11p110-overexpressing EC109 and KYSE150 cell lines. All the data are presented as the mean ± SD (n = 3) and comparisons among three groups were analyzed by one-way ANOVA and comparisons between two groups were performed by student’s t test.