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. 2019 Feb 12;18(4):437–451. doi: 10.1080/15384101.2019.1577650

Figure 4.

Figure 4.

TRPS1 regulated mouse ZGA and histone H3 trimethylation in two-cell embryos. (a) Effects of Trps1-siRNA on the mRNA expression of ERα, eIF1A, MuERV-L, Zscan4d and Hsp70.1. About 140 embryos were used in each experimental group (N = 6). (b and d) Trps1 knockdown resulted in down-regulation of H3K4me3 (b) and H3K27me3 (d) in both mid and late two-cell embryos. (c and e) Gray value analysis results for (b) and (d), respectively. Mid two-cell, 20 h post injection; late two-cell, 29 h post injection. About 80 embryos were used in each experimental group (N = 3). * P < 0.05, ** P < 0.01, ***P < 0.001, Student’s t-test. Error bars indicate SEM. Scale bar, 20 μm.