Figure 5.

TRPS1 differentially regulated the expression of Sox2 and Oct4. (a and b) Trps1-siRNA significantly changed the mRNA expression level of Sox2 (a) and Oct4 (b). About 60 embryos were used in each experimental group (N = 3). (c, d and e) Trps1 knockdown resulted in less nuclear localization of SOX2 protein at late two-cell stage (C and E), but no change was observed in early two-cell embryos (c and d). (f, g and h) Trps1 knockdown resulted in elevated cytoplasm expression level of OCT4 protein at mid (f and g) and late (f and h) two-cell stage. Mid two-cell, 20 h post injection; late two-cell, 29 h post injection. About 80 embryos were used in each experimental group (N = 3). * P < 0.05, ** P < 0.01, ***P < 0.001, Student’s t-test. Error bars indicate SEM. Scale bar, 20 μm.