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. 2018 Nov 7;20(4):552–561. doi: 10.1080/15384047.2018.1538613

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Figure 4. — ARAP1-AS1 acted as a ceRNA to bind miR-4735-3p.

A. The localization of ARAP1-AS1 was identified in J82 and T24 cells with subcellular fractionation assay. B. Top ten miRNAs which can bind with ARAP1-AS1 were predicted and obtained from DIANA. C. MS2-RIP assay was carried out to determine which one of those ten miRNAs can bind with ARAP1-AS1 in BCa cells. D. The luciferase activity analysis was utilized to demonstrate the binding relation between ARAP1-AS1 and two candidate miRNAs. E. The binding sequence between the wild type ARAP1-AS1 (ARAP1-AS1-WT) or mutant type ARAP1-AS1 (ARAP1-AS1-MUT) and miR-4735-3p was obtained and illustrated. F. Further luciferase reporter assay was performed to confirm the combination between ARAP1-AS1 and miR-4735-3p. G. The level of ARAP1-AS1 was observed in BCa tissues using qRT-PCR. H. The expression relevance between ARAP1-AS1 and miR-4735-3p in BCa tissues was analyzed. I. The expression change of miR-4735-3p was detected in APAP1-AS1-dwonregulated BCa cells. **p < 0.01 vs. control group. N.S: no significance.