Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Pseudomonas
aeruginosa) |
crc | NA | Pseudomonas genome database: PA5332 |
|
| Gene (P. aeruginosa) | amiE | NA | Pseudomonas genome database: PA3366 |
|
| Strain, strain background (P. aeruginosa) |
PAO1 | PMID: 111024 | ||
| Strain, strain background (P. aeruginosa) |
PAO1Δcrc | PMID: 20080802 | ||
| Strain, strain background (Escherichia coli) |
XL1-Blue | Stratagene | ||
| Strain, strain background (E. coli) |
BL21(DE3) | Novagen | ||
| Genetic reagent (P. aeruginosa) |
amiE6ARN RNA | Microsynth, PMID: 29244160 |
sequence: 5'-AAAAAUAACAACAAGAAG-3' |
|
| Antibody | anti-Crc (rabbit polyclonal) |
Pineda | (1:5000) | |
| Antibody | anti-Hfq (rabbit polyclonal) |
Pineda | (1:10000) | |
| Antibody | anti-S1 (rabbit polyclonal) |
Pineda | (1:10000) | |
| Antibody | anti-rabbit IgG (goat polyclonal) |
Sigma | Sigma: A3687 | conjugated with alkaline phosphatase, (1:10000) |
| Recombinant DNA reagent |
pME9655 (plasmid) |
PMID: 20080802 | ||
| Recombinant DNA reagent |
pETM14lic-His6Crc (plasmid) |
PMID: 23717639 | ||
| Recombinant DNA reagent |
pME4510crcFlag
(plasmid) |
PMID: 29244160 | ||
| Peptide, recombinant protein |
Hfq | PMID: 21330354 | ||
| Peptide, recombinant protein |
Crc | PMID: 23717639 | ||
| Software, algorithm | Relion3 | DOI: 10.7554/eLife.42166 | ||
| Software, algorithm | LocScale | DOI: 10.7554/eLife.27131 | ||
| Software, algorithm | LocalDeblur | DOI: 10.1101/433284 |