Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 7;18(4):395–412. doi: 10.1080/15384101.2018.1564492

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Informa UK Limited, trading as Taylor & Francis Group

PMC Copyright notice

Figure 7. — PKCβ1 knockdown disrupted the metaphase-anaphase transition of mouse oocytes as revealed by time-lapse live cell imaging. (a)Oocytes were co-injected with β5-tubulin-GFP mRNA, H2B-RFP mRNA and control siRNA. Spindle (fluorescent tubulin) and DNA (red) images in a representative control oocyte during in vitro maturation. Time points indicating the time lapse from GVBD occurrence in the oocytes. (b)Similar to (A), oocytes were co-injected with β5-tubulin-GFP mRNA, H2B-RFP mRNA and PKCβ1 siRNA. Representative images showing the PKCβ1 depleted oocytes with abnormal spindles, Pro-MI/MI arrested chromosomes, misaligned chromosomes, repetitive and unsuccessful chromosome segregation and PB1 extrusion failure. Green, tubulin; red, DNA. Bar = 20μm.