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. 2019 Feb 26;10(1):166–179. doi: 10.1080/21505594.2019.1582975

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Determination of antigen-specific sIgA and IgG antibody levels in mice immunized with pPG-α/L. casei 393. (a) The immunization scheme, and the schedule of feces, serum, and intestinal mucus sampling. After immunization, immune samples were collected from the mice in each group on days 0, 7, 14, 21, 28, 35, and 42, and anti–α-toxoid sIgA levels in the intestinal mucus (b) and feces (c) and anti–α-toxoid IgG levels in serum (d) were detected by an ELISA. Bars represent the mean ± standard error in each group (*p < 0.05, **p < 0.01 as compared with groups “pPG/L. casei 393” and “PBS”).