Table 1.
Plasmids | Relevant Characteristics | Description/Reference |
---|---|---|
pMD19-T | A cloning vector; ampicillin resistance, Ampr | Takara, China |
pMD19-T-α | Recombinant pMD19-T containing a gene encoding the toxoid of C. perfringens α-toxin; Ampr | In this work |
pET-32a | A vector for prokaryotic expression; Ampr | Takara, China |
pET-32a-α | Recombinant pET-32a containing a gene encoding the toxoid of C. perfringens α-toxin for α-toxoid expression to develop monoclonal antibodies; Ampr | In this work |
pPG-T7g10 | A constitutive expression plasmid consisting of the HCE promoter, PgsA anchor, rrnBT1T2 terminator, and repA and repC replicon; chloramphenicol resistance (Cmr) gene | [24] |
pPG-α | Recombinant pPG-T7g10 containing the gene encoding toxoid of C. perfringens α-toxin for constructing the genetically engineered Lactobacillus casei; Cmr | In this work |