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. 2019 Feb 26;10(1):166–179. doi: 10.1080/21505594.2019.1582975

Table 1.

Details of plasmids used in this study.

Plasmids Relevant Characteristics Description/Reference
pMD19-T A cloning vector; ampicillin resistance, Ampr Takara, China
pMD19-T-α Recombinant pMD19-T containing a gene encoding the toxoid of C. perfringens α-toxin; Ampr In this work
pET-32a A vector for prokaryotic expression; Ampr Takara, China
pET-32a-α Recombinant pET-32a containing a gene encoding the toxoid of C. perfringens α-toxin for α-toxoid expression to develop monoclonal antibodies; Ampr In this work
pPG-T7g10 A constitutive expression plasmid consisting of the HCE promoter, PgsA anchor, rrnBT1T2 terminator, and repA and repC replicon; chloramphenicol resistance (Cmr) gene [24]
pPG-α Recombinant pPG-T7g10 containing the gene encoding toxoid of C. perfringens α-toxin for constructing the genetically engineered Lactobacillus casei; Cmr In this work