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. 2018 Nov 7;20(4):444–453. doi: 10.1080/15384047.2018.1529119

Figure 4.

Figure 4.

STAT3 is a transcription activator of HNF1A-AS1. A. The expression of STAT3 in OSCC tissues was examined by qRT-PCR. B. The relevance between the expression of STAT3 and that of HNF1A-AS1 in OSCC tissues was analyzed by Pearson correlation analysis. C. The levels of STAT3 and p-STAT3 were detected in OSCC cells and normal cell line. D. The mRNA level of STAT3 in OSCC cell lines and one normal cell line was detected with qRT-PCR. E. The expression of HNF1A-AS1 was examined in response to STAT3 overexpression or knockdown. F. The motif of STAT3 binding sequence was obtained from JASPAR. G. Top five binding sites of STAT3 in part 1 (P1) and part 2 (P2) of HNF1A-AS1 promoter were predicted and obtained from JASPAR. H. ChIP assay further demonstrated the affinity of STAT3 to the part 1 (P1) of HNF1A-AS1 promoter. I. The luciferase reporter assay was conducted to determine the binding condition of three sites of P1. J. The levels of STAT3 and p-STAT3 were detected in cells transfected with si-JAK1 or si-JAK2. K. The luciferase reporter assay was conducted in two OSCC cells transfected with STAT3 expression vector and si-JAK1. **P < 0.01 vs. control group.