Table 2.
Hypothetical RFLP lengths for CYP3A4*4, CYP3A4*18B and CYP3A4*22 following digestion with BsmAI, RsaI and BseYI, respectively
| SNPs | PCR sizes | RE and the recognition sites | REs tested | Frequencies | Fragments (bp) |
|---|---|---|---|---|---|
| CYP3A4*4 | 244 | BsmAI | |||
| Wild type | 5'...G T C T C (N)1↓...3' | BsmAI | 2 | 15, 88, 141 | |
| 3'...C A G A G (N)5↑ ...5' | RsaI | ND | 331 | ||
| BseYI | ND | 793 | |||
| Mutant | BsmAI | 3 | 15, 47, 88, 94 | ||
| RsaI | ND | 331 | |||
| BseYI | ND | 793 | |||
|
| |||||
| CYP3A4*18B | 331 | RsaI | |||
| Wild type | 5'…G T ↓ A C …3' | RsaI | 1 | 115, 216 | |
| 3'…C A ↑ T G …5' | BsmAI | ND | 244 | ||
| BseYI | ND | 793 | |||
| Mutant | RsaI | ND | 331 | ||
| BsmAI | ND | 244 | |||
| BseYI | ND | 793 | |||
|
| |||||
| CYP3A4*22 | 793 | BseYI | |||
| Wild type | 5'…C ↓ C C A G C …3' | BseYI | 1 | 219, 574 | |
| 3' …G G G T C ↑ G …5' | BsmAI | 3 | 56, 59, 153, 525 | ||
| RsaI | 1 | 112, 618 | |||
| Mutant | BseYI | ND | 793 | ||
| BsmAI | 3 | 56, 59, 153, 525 | |||
| RsaI | 1 | 112, 681 | |||
ND: no digestion