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. 2018 Nov 28;196(1):39–51. doi: 10.1111/cei.13242

Figure 6.

Figure 6

Toll‐like receptor (TLR)‐7 signal activation in salivary gland epithelial cells (SGECs) and peripheral blood mononuclear cells (PBMCs) from the primary Sjögren's syndrome (pSS) patients. (a) The levels of interferon (IFN)‐α in SGECs and PBMCs from pSS patients (n = 6) stimulated with 1 mM loxoribine for 6 h and/or 1000 U/ml IFN‐β for 12 h measured by enzyme‐linked immunosorbent assay (ELISA). (b) The expressions of IFN‐α and TLR‐7 in CD123+CD303+ pDCs from pSS patients (n = 6) stimulated with 1 mM loxoribine for 6 h and/or 1000 U/ml IFN‐β for 12 h, analyzed by flow cytometry. ** P < 0·01 by analysis of variance (anova). Error bars: standard deviation. (c) The correlation between the mean fluorescence intensity (MFI) of IFN‐α or TLR‐7 stains in mononuclear cells of LSGs and the percentage of IFN‐α+TLR‐7+ pDCs in flow cytometry from pSS patients (n = 6). The MFI of immunostaining was captured and calculated with a hybrid cell count system. The correlations were determined using Spearman's rank correlation coefficient. P‐values < 0·05 were considered significant; n.d. = not detected.