Figure 2.

MoImd4 is required for pathogenicity. (A) Conidial suspensions (5 × 10⁴ spores/mL) of Guy11, ∆Moimd4 and the complemented strains were sprayed onto 11‐day‐old rice seedlings and photographs were taken at 7 days post‐inoculation (dpi). (B) Conidial suspensions (15 × 10⁴ spores/mL) were injected into 18‐day‐old rice sheaths and photographs were taken at 5 dpi. (C) Lesion type statistical analysis (0, no lesion; 1, pinhead‐sized brown specks; 2, 1.5‐mm brown spots; 3, 2–3‐mm grey spots with brown margins; 4, many elliptical grey spots longer than 3 mm; 5, coalesced lesions infecting 50% or more of the leaf area). Lesions were photographed and measured at 7 dpi. Experiments were repeated three times with similar results. Asterisks represent significant differences (Duncan’s new multiple range test, P < 0.01). (D) The severity of lesions was analysed by quantification of the Magnaporthe oryzae genomic 28S rDNA relative to rice genomic Rubq1 DNA. Experiments were repeated three times with similar results. Error bars represent the standard deviations and asterisk represents significant difference (Duncan’s new multiple range test, P < 0.01).