Figure 3.

Xanthosine monophosphate (XMP) levels are critical for the growth and virulence of Magnaporthe oryzae. (A) Guy11, ∆Moimd4 and the complemented strains were co‐incubated on minimal medium (MM) treated with or without 1, 2.5 and 5 mM XMP at 28 °C in the dark for 7 days. White arrowheads represent the edges of mycelia. (B) The indicated strains were co‐incubated on MM with or without 1, 5 and 10 µg/mL mycophenolic acid (MPA) at 28 °C for 7 days in the dark. White arrowheads represent the edges of mycelia. (C) Four types of IHs in rice sheath cells; the four different shapes are shown in the bottom right‐hand corner: type 1, no penetration; type 2, a single invasive hypha; type 3, extensive hyphal growth in only one rice cell; type 4, extensive hyphal growth in neighbouring rice cells. Statistical analysis of the type of IH of the indicated strains, treated with or without 1 mM XMP or 10 µg/mL MPA at 48 h; approximately 100 IHs were counted and the experiments were repeated three times with similar results. The error bars indicate the standard deviations of three replicates. Bar, 10 μm.