Table 2.
Turgor assays and appressorium formation of the wild type, ∆Moimd4 mutant, and the complement strains.
| Strain | Appressorium exhibiting incipient cytorrhysis (%)† | Appressorium formation (%)‡ | ||||
|---|---|---|---|---|---|---|
| 1 m | 2 m | 3 m | 4 m | Hydrophobic | Hydrophilic | |
| Guy11 | 30.7 ± 5.0 | 52.0 ± 5.7 | 67.0 ± 7.1 | 83.3 ± 4.2 | 99.1 ± 2.2 | 0 |
| ∆Moimd4 | 42.0 ± 3.2* | 65.0 ± 2.0* | 80.0 ± 2.1* | 92.0 ± 2.6* | 98.5 ± 2.8 | 0 |
| MoImd4 | 34.7 ± 4.2 | 56.0 ± 5.5 | 72.0 ± 1.5 | 79.4 ± 3.2 | 99.5 ± 3.2 | 0 |
†Different concentrations of glycerol (1 to 4 m) were used to analyse incipient cytorrhysis. At least 100 appressoria were counted for each concentration.
‡Appressorium formation on hydrophilic or hydrophobic surfaces at 24 h post incubation; ±SD was calculated from three repeated experiments and asterisks indicate statistically significant differences (Duncan’s new multiple range test, *means P < 0.01).